This page explains what the rules say. It gives no advice on passing a test.
Some jobs are covered by a detailed federal testing rulebook. Most jobs are not.
Which world you are in changes everything below.
The federal rulebook described here is 49 CFR Part 40, from the U.S. Department of Transportation.
Part 40 sets the procedures for drug and alcohol testing of safety-sensitive transportation workers — jobs like truck and bus drivers, airline pilots, and mariners.
The standard test reasons listed in Part 40 (§ 40.14) are:
A pre-employment test is one where a negative result is required before the person can perform safety-sensitive work. Section 40.23(g) treats pre-employment as a test where “a negative result is required.”
The DOT drug panel is:
Plus four semi-synthetic opioids:
A lab “positive” is not the last step.
A Medical Review Officer (MRO) — a licensed physician — interviews the person to look for a legitimate medical explanation, such as a valid prescription.
If a legitimate medical explanation is established, the MRO reports the result to the employer as NEGATIVE.
If not, the MRO reports it as POSITIVE.
Before the MRO reports certain medication or medical information to a third party, the person has up to five days to have their prescribing physician contact the MRO.
A verified positive means the employer must immediately remove the person from safety-sensitive functions (§ 40.23(a)).
A verified adulterated or substituted result counts as a refusal to test, with the same immediate removal (§ 40.23(b)).
Part 40 also provides for a split specimen: the employer may not wait for a split-specimen result before acting (§ 40.23(a) names the split specimen test).
The way back is a set process, and it applies before safety-sensitive work for ANY DOT-regulated employer (§ 40.23(d) and the DOT notice):
There is no single federal rulebook like Part 40 for most private employers.
Outside the DOT world, testing is mostly a mix of company policy and state law — and state laws vary widely from state to state.
One federal law still matters: the Americans with Disabilities Act (ADA), enforced by the EEOC. What the EEOC says:
Marijuana is still on the federal DOT panel (Part 2 above), whatever a state’s own marijuana law says — and state laws on marijuana and testing vary.
Whether any one person keeps or gets any one job depends on which rulebook applies, the employer’s own policy, and state law.
This page states the rules only.